Journal: Frontiers in Veterinary Science

Article Title: Role of Animal Models to Advance Research of Bacterial Osteomyelitis

doi: 10.3389/fvets.2022.879630

Figure Lengend Snippet: Rat models of osteomyelitis.

Article Snippet: Wu et al. ( ) , Virulence of methicillin-resistant Staphylococcus aureus modulated by the YycFG two-component pathway in a rat model of osteomyelitis , Female, Sprague-Dawley , 260–280 g , 4 weeks , MRSA (clinical strain) and ASyycG over- expression MRSA clinical strain (ASyycG mutant). 40 μl of mid-exponential phase , Injection into intramedullary canal , Drill defect to antero-medial tibia with medullary cavity exposure , In vivo microCT, histology, SEM, rtPCR.

Techniques: Injection, Comparison, Imaging, In Vitro, In Vivo, Infection, Animal Model, Gypsum, Formulation, Activity Assay, Membrane, Modification, CRISPR, In Situ, Over Expression, Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Drug discovery, Biomarker Discovery, Immunohistochemistry

Journal:

Article Title: MC21-A, a Bactericidal Antibiotic Produced by a New Marine Bacterium, Pseudoalteromonas phenolica sp. nov. O-BC30 T , against Methicillin-Resistant Staphylococcus aureus

doi: 10.1128/AAC.47.2.480-488.2003

Figure Lengend Snippet: Comparative bactericidal activities of MC21-A and vancomycin against a reference strain of MRSA ATCC 33591 (A) and a clinical isolate of MRSA, E 31243 (B). •, growth control; □, MIC; ▵, two times the MIC; ×, four times the MIC; ○, eight times the MIC. The values with standard error bars are mean values from duplicate experiments.

Article Snippet: The following strains were used to evaluate the antibacterial activities of MC21-A: 10 clinical isolates of MRSA (strains E 31224, E 31237, E 31243, E 31256, E 31271, E 31280, and E 31283, kindly provided by S. Araki, Eisai Co. Ltd., Tokyo, Japan), strain 7B29 (kindly provided by T. Someya, Saga University, Saga, Japan), and strains GIFU 12361 and GIFU 12364 (kindly provided by H. Yamamoto, Gifu University, Gifu, Japan); a reference strain of MRSA (ATCC 33591); two strains of methicillin-sensitive S. aureus (MSSA; IFO 15035 and ATCC 25923); one strain of Bacillus subtillis (IFO 14419); three strains of Enterococcus serolicida (E 9053, E 9568, and NG 8206, kindly provided by S. Araki, Eisai Co. Ltd.); three strains of E. faecium (NBRC 3128, NBRC 3535, and NBRC 3826); three strains of E. faecalis (NBRC 3971, NBRC 3989, and NBRC 12964); and Streptococcus mutans (NBRC 13955), Streptococcus pneumoniae (GTC 261), and Streptococcus pyogenes (GTC 262) (kindly provided by the Gifu Type Culture Collection, Department of Microbiology, Gifu University).

Techniques: Control

Journal:

Article Title: MC21-A, a Bactericidal Antibiotic Produced by a New Marine Bacterium, Pseudoalteromonas phenolica sp. nov. O-BC30 T , against Methicillin-Resistant Staphylococcus aureus

doi: 10.1128/AAC.47.2.480-488.2003

Figure Lengend Snippet: Bacteriolytic activities of MC21-A against a reference strain of MRSA (ATCC 33591) and a clinical isolate of MRSA (E 31243). •, positive control (1 μg of lysostaphin per ml); ○, the MIC; ▵, two times the MIC; □, four times the MIC. Relative absorbance was calculated by dividing the absorbance for the treated tube by that for the negative control tube. The values with standard error bars are mean values from triplicate experiments.

Article Snippet: The following strains were used to evaluate the antibacterial activities of MC21-A: 10 clinical isolates of MRSA (strains E 31224, E 31237, E 31243, E 31256, E 31271, E 31280, and E 31283, kindly provided by S. Araki, Eisai Co. Ltd., Tokyo, Japan), strain 7B29 (kindly provided by T. Someya, Saga University, Saga, Japan), and strains GIFU 12361 and GIFU 12364 (kindly provided by H. Yamamoto, Gifu University, Gifu, Japan); a reference strain of MRSA (ATCC 33591); two strains of methicillin-sensitive S. aureus (MSSA; IFO 15035 and ATCC 25923); one strain of Bacillus subtillis (IFO 14419); three strains of Enterococcus serolicida (E 9053, E 9568, and NG 8206, kindly provided by S. Araki, Eisai Co. Ltd.); three strains of E. faecium (NBRC 3128, NBRC 3535, and NBRC 3826); three strains of E. faecalis (NBRC 3971, NBRC 3989, and NBRC 12964); and Streptococcus mutans (NBRC 13955), Streptococcus pneumoniae (GTC 261), and Streptococcus pyogenes (GTC 262) (kindly provided by the Gifu Type Culture Collection, Department of Microbiology, Gifu University).

Techniques: Positive Control, Negative Control

Journal:

Article Title: MC21-A, a Bactericidal Antibiotic Produced by a New Marine Bacterium, Pseudoalteromonas phenolica sp. nov. O-BC30 T , against Methicillin-Resistant Staphylococcus aureus

doi: 10.1128/AAC.47.2.480-488.2003

Figure Lengend Snippet: Cells membrane permeabilization of MRSA induced by MC21-A (A), chloramphenicol (B), amoxicillin (C), and vancomycin (D). ▵, untreated control; □, 1/16 the MIC; ○, 1/4 the MIC; ▪, the MIC; •, 4 times the MIC. The MICs of chloramphenicol and amoxicillin were each 64 μg/ml. The fluorescence values with standard error bars are the corrected mean values from quadruplet measurements. The corrected fluorescence values are each fluorescence value subtracted from the background value.

Article Snippet: The following strains were used to evaluate the antibacterial activities of MC21-A: 10 clinical isolates of MRSA (strains E 31224, E 31237, E 31243, E 31256, E 31271, E 31280, and E 31283, kindly provided by S. Araki, Eisai Co. Ltd., Tokyo, Japan), strain 7B29 (kindly provided by T. Someya, Saga University, Saga, Japan), and strains GIFU 12361 and GIFU 12364 (kindly provided by H. Yamamoto, Gifu University, Gifu, Japan); a reference strain of MRSA (ATCC 33591); two strains of methicillin-sensitive S. aureus (MSSA; IFO 15035 and ATCC 25923); one strain of Bacillus subtillis (IFO 14419); three strains of Enterococcus serolicida (E 9053, E 9568, and NG 8206, kindly provided by S. Araki, Eisai Co. Ltd.); three strains of E. faecium (NBRC 3128, NBRC 3535, and NBRC 3826); three strains of E. faecalis (NBRC 3971, NBRC 3989, and NBRC 12964); and Streptococcus mutans (NBRC 13955), Streptococcus pneumoniae (GTC 261), and Streptococcus pyogenes (GTC 262) (kindly provided by the Gifu Type Culture Collection, Department of Microbiology, Gifu University).

Techniques: Membrane, Control, Fluorescence